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Customer Profiles High Pressure Systems
Meristemal Propagation


VitroPlant Klosterneuburg

VitroPlant propagates agricultural and ornamental plants
by means of meristemal techniques

Micro-propagation of meristemes is nowadays a proven technology, however the most critical phase is the day of extraction, when the plantlets are taken out from the ideal micro-climate of the sterile propagation flask, to be rooted in substrate and to grow to a deliverable plant. To support the fragile plantlets during this transitions phase a fogging plant is of utmost importance to reduce the dropouts to a low enough level.

Mr. DI Leonhard, one of the managers at VitroPlant extended the specifications for plantlet hardening one step further than usual. Following the first step of hardening and rooting the humidity of the propagation table should be re-adjusted without moving the table to another location to complete the hardening phase - i.e. final rooting and hardening without fogging.

Double-decker climate chambers - at the lower level the meristemes upper level hardening chambers where the plantlets are acclimatisized to non-sterile surroundings

To fulfil these specifications with a total of some 24 table being in use at any point in time we have configured the system as follows :
The system consists of two independent high-pressure aggregates.
Each of them feed two pressure lines to the row of tables on both sides of the hardening house. Each of the tables contains only one nozzle-line - set with nozzles type K1-150 - has however two feeder lines with manual valves.
A humidity-feeler - representing all tables on one side of the house - is positioned in one of the tents covering the tables and set to the desired (high) value for phase 1. A second feeler detects the humidity for phase 2 (usually at a lower humidity value) in a hardening tent. If necessary the feelers can be placed in each of the tents having connection cables long enough. The other side of the house is configured similarly.

To illustrate the method - assumes tables 1, 2, 6, 9 and 12 in the left row receive fresh plantlets from the in-vitro-cultures. They receive a humidity of some 95% relative. The feeler is placed in the tent of table 6, which represents tables 1, 2, 9 and 12. Pump 1 with feeder line 1 serves these tables according to the controls.
The manual valves of feeder line 1 are opened to the a.m. tables; the valves to feeder line 2 remain closed.

Details of installation: each of the lower table-levels is connected to both feeder lines. Switching from one to the other feeder line is done manually by opening one valve and closing the other.

The tables 3, 4, 5, 10 and 11 contain larger plantlets; their feeler is placed in table 5 and thereby represents all other connected tables. The feeder lines are serviced by pump 2 with line 2, therefore the valves are open for line 2, line 1 remains closed. The rest of the tables (7 and 8) are currently not in use, being empty. Both valves to the feeder lines remain closed.

The system was installed in 1988 and has been running with fullest satisfaction since then
Information from DI Leonhard, Tel. +43 - 2243 32 698

Double Pump-Stand

Main manifold with 4 high-pressure valves.
The lyra-bent piping provides a possibility to resort manually to single-pump operation in case one of the pumps fails temporarily.


Customer Profiles High Pressure Systems
Meristemal Propagation



last modified MAR 2013
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